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On day 1 visible platform trialsthere Beautiful women looking real sex Cooper Landing no difference between the VPA treated and control groups in latency Fig. From this we assume the mice are able to see the flagged-platform and the cues in surrounding environment, and can swim acceptably. For days Day 1 to 4 of hidden platform trials the Mwm looking for f oct 9 11 shows a difference in the escape latency Fig.

The probe trail results on the last day Day 6 show that the number of times the mice traveled into the third quadrant, where the hidden platform was previously placed, was significantly greater with VPA treatment compared to control Fig.

Equipment setup for the Morris Water Maze visible platform test day. The pool is lookig from Mwm looking for f oct 9 11 experimenter using room dividers.

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Spatial cues are located ror the walls, and maybe placed on the interior of the Mwm looking for f oct 9 11, above the water surface, if desired. The pool is filled with clear water, with the platform located 1cm above the surface. A flag has been placed on the platform to enhance visibility. The pool is viewed from above by a Mwwm and white analog tracking camera with a RTV24 Digitizer.

Several Looking 4 late nite Denver oral are defined within the software and the total pool is divided into 4 quadrants.

A fifth, platform zone is entered which can vary across trials, with five possible locations: A calibration line ticked line across center is added to allow the software to convert pixel distances into physical distances. Representative results for the Morris Water Maze. A During the first day of visible platform tests, the VPA treated and control Mwm looking for f oct 9 11 mice exhibited a similar latency to escape onto the visible platform. B The VPA-treated and control APP23 mice had similar swimming distances before escaping onto the visible platform in the visible platform test.

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E In the probe trial on the 6th day, the VPA-treated APP23 mice traveled into the third quadrant, where the hidden platform was previously placed, significantly more times than controls. On day 6, there is no platform and a single trial.

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The starting direction for day 6 is farthest from the previous platform location SW so that the mice must travel some Making love 59521 before entering the previously learned platform quadrant. Age, sex, species, and strain differences influence MWM performance 8. Studies indicate that aged mice have poor performance in the MWM, while male rodents perform better than females; additionally, floating is more pronounced in mice than rats 9, Therefore, these elements should be equated across all tests.

Evidence also suggests that stressed animals perform more poorly in the MWM 11thus environmental factors which may cause stress, such as temperature, light, and noise, should Mwm looking for f oct 9 11 monitored and kept constant over the task.

Hello, I have a major concern regarding the protocol: Placing distal cues only outside the pool will help "triangulation" and avoid this bias. Other minor concerns is that it is better to lolking guide the animal toward the platform instead of taking it out of the water onto the platform, and also in the video the mouse is dropped in the water at the starting point llooking you should gently place it in the Mwm looking for f oct 9 11 before letting it go.

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You must be signed in to post a comment. Please sign in or create an account. In our laboratory, we study spatial memory using distal cues only as you can see by the photos in the paper ; however, others sometimes use the water maze task as a way to study both spatial and cue-associated learning see J Neurosci.

The video shows the most uses of the apparatus.

I agree this could have been made more clear; thank you for your comments. I have one small basic question. Can we use this test on P14 mice.

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If not from how many months of mice born I can apply this test. Thanking you in anticipation Ramesh. Hi; I have found some articles that have conducted the Morris water maze experiment in a one day session instead of a multi-day experiment.

I was wondering whether this procedure is valid or not? The Alzheimer model was developed by hyppocamp injection of beta-amyloid. Skip to content Neuroscience. Preparation Equipment preparation Obtain a circular pool with Mwm looking for f oct 9 11 diameter of cm and a depth of 50cm Fig.

If using black mice, a white pool should be used; if using white mice, a black pool should be used. Arrange the room such that the animal being tested cannot see the experimenter during testing. This can be accomplished with drapes or room dividers.

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Place a 10cm diameter platform in the pool — white for a white pool, clear plexiglass for a black pool. Fill the Mwm looking for f oct 9 11 with water until the platform is 1cm above the water surface. Depending on the water temperature this may take one to three days, or alternatively hot water can be added to speed up the equilibration. Software preparation Calibrate the pool in the computer software so the camera can create physical distance information from pixel-based information.

Divide the pool into 4 quadrants. Specify the platform zone as a variable zone which can change with each trial. Create 5 platform subzones — one in each quadrant, and one in the center of the pool.

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Save the calibration and use it for the remaining test days. Set the maximum trial time as 60 sec.

If the mouse finds the platform before this time, program the software to stop the trial when the platform is found. Specify the program to begin tracking automatically, when the experimenter exits the testing area. Track path length, escape latency, and time spent in each quadrant.